Abstract
Ionic liquids (ILs)-resistant cellulase enzymes can facilitate the saccharification of IL-pretreated biomass in a one-pot wash-free method. Using a bioinformatics approach, two cellulases, Persicel7 and Persicel8, with convincing evidence for ion liquid tolerance were identified. Subsequently, these enzymes were heterologously expressed and biochemically characterized. Persicel7 and Persicel8 exhibited endo-β-1, 4-glucanase activity and were resistant to inhibitors and several organic solvents. Thier activity in 10% (v/v) 1-Ethyl-3-methylimidazolium chloride and 1-Butyl-3-methylimidazolium chloride were higher than 130%, as compared with IL-free control. The half-life of cellulases improved up to 11-fold when incubated with 20% (v/v) solution of ion liquids. A one-pot IL-pretreatment and enzymatic saccharification process based on Persicel7 and Persicel8 were established. By this process, the saccharification rate of rice straw was enhanced by 33% versus the untreated reaction. The Persicel7 and Persicel8 unique properties make them attractive candidates for industrial applications, particularly hydrolyzing ion liquid activated biomass in a one-pot system.
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