Abstract
An l-arabinose-inducible T7 expression system was shown to be highly stringent in the regulation of cloned gene expression. However, this stringency was medium-dependent and missing in complex media. To ensure consistent expression efficiency, the system was improved by constructing T7 lac promoter-containing plasmids subject to the control of thermo-labile lacI. As a result, LacZ was produced to reach a 128-fold increase over the uninduced level in rich media. Furthermore, a large production of carbamoylase using the developed system was successfully achieved in a lab-scale fermenter. The production of soluble protein was 10% of total cell protein content and the cell yield 30 g dry cell weight per litre. Taken together, these illustrate the great promise of this system for industrial use.
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