Abstract
We demonstrate a novel strategy to produce (R)-3-hydroxybutyric acid [(R)-3-HB] using Pseudomonas sp. DS1001a or its extracellular poly (3-hydroxybutyrate) (PHB) depolymerase. While approximately 77.8% PHB was degraded by Pseudomonas sp. DS1001a after 12h of cultivation, only 1.555g/l of 3-HB monomer was produced because of assimilation by the strain. About 8.58g/l (R)-3-HB was obtained after 8h of incubation using extracellular PHB depolymerase from Pseudomonas sp. DS1001a. The optimal temperature and pH of PHB depolymerase were 50°C and 8, respectively. CaCO3 increased (R)-3-HB yields to 23.97g/l by stabilizing the pH of the reaction system. High yields of the (R)-3-HB product and the low cost of the medium and CaCO3 indicate the feasibility of bioconversion of low-value PHB waste or industrial leftovers into high-value (R)-3-HB using extracellular PHB depolymerase.
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