Abstract

Lactic acid has been identified as one of the top 30 potential building-block chemicals from biomass. Therefore, the search for cheap raw materials is an objective to reduce the production costs. Efficient polymer-grade L-lactic acid production was achieved in this report by a thermophilic strain Bacillus sp. XZL4 using corn stover hydrolyzate as sole carbon source. High L-lactic acid concentration (81.0 g L-1) was obtained from 162.5 g L-1 concentrated corn stover hydrolyzate (total reducing sugar of 83.0 g L-1) with a volumetric productivity of 1.86 g L-1 h-1 (0–36 h) and a product yield of 0.98 g g-1 total reducing sugars. This is the highest L-lactic acid concentration and yield reported from corn stover hydrolyzate. And the high optical purity of L-lactic acid obtained in this study also indicated that Bacillus sp. XZL4 is a promising polymer-grade L-lactic-acid producer from cellulosic biomass.

Highlights

  • Lactic acid is a valuable chemical and one of its extensive applications is for polymerization of L-lactic acid to poly (L-lactic acid), which is an attractive polymer because it can be produced from renewable resources and is biodegradable

  • The batch fermentation results of high yield and high optical purity of L-lactic acid from corn stover hydrolyzate indicated that Bacillus sp. strain XZL4 used in this study is a promising L-lactic-acid producer from cellulosic biomass

  • It is notable that Bacillus sp. strain XZL4 could utilize both the two sugars simultaneously, glucose was utilized a little faster than xylose

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Summary

Introduction

Lactic acid is a valuable chemical and one of its extensive applications is for polymerization of L-lactic acid to poly (L-lactic acid), which is an attractive polymer because it can be produced from renewable resources and is biodegradable These properties have strengthened interest in developing more efficient production processes for optical purity of L-lactic acid (Wang et al 2010a). To improve productivity and economy of lactic acid production, some reports have investigated the potential of utilizing low-cost raw materials as carbon sources, such as molasses and cellulosic materials (Patel et al 2004; Romaní et al 2008; Wang et al 2010a). The batch fermentation results of high yield and high optical purity of L-lactic acid from corn stover hydrolyzate indicated that Bacillus sp. strain XZL4 used in this study is a promising L-lactic-acid producer from cellulosic biomass

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