Abstract
The effect of immobilizing Gluconobacter oxydans WSH-003 on a novel combination carrier material to produce l-sorbose was investigated. A combination carrier of sodium alginate and diatomite was shown to be biocompatible and durable. Our results showed that the carrier material concentration and bead diameter of immobilized cells significantly affected l-sorbose yield. Optimal medium conditions for fermenting l-sorbose were determined to be concentrations of 2.5% (w/v) sodium and 0.5% (w/v) diatomite as well as 2.0-mm bead diameter. Under optimal conditions, fermentation time was 1.3 times lower compared to previous experiments involving free cells. Additionally, the immobilized cells showed excellent stability and were maintained for more than 15 days in a bioreactor. l-sorbose yield was more than 81.0% in the previous nine runs of repeated-batch fermentation. These results indicate that maintaining highly active immobilized cells is feasible for the biosynthesis of l-sorbose.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
