Efficient production of induced double haploid (DH) plantsderived from anther culture

Abstract

The success of DH technology dependents on the efficacy of optimized protocol that could be utilized for large scale production of homozygous lines. In this study, anther culture protocol was established using invitro colchicine treatment of haploid callus derived from anthers of niger (Guizotia abyssinica L.F. cass). Flower buds at uninucleate stage were kept for 24 hours cold pre-treatment followed by anther inoculation. 30-40 days old callus were subjected to different colchicine treatments (0, 0.025%, 0.05%, 0.1%, 0.2%) for different time intervals (6,16, 24, 30, 48hours). Colchicine treated (CT) callus were transferred to greening media followed by transferring to shooting media. Observations of greening and shooting percentage were taken with reference to control. The greening percentage found to be decreased (34%) compared to control (40%). However, 90-95% and 85-90% increment in shooting and rooting percentage was observed at 0.2% CT for 30 hours as compared to control which showed 15-20% shooting and 40-45% rooting. It was concluded that the incorporation of CT at callus stage increase the overall efficiency of DH production.