Efficient polyethylene terephthalate biodegradation by an engineered Ideonella sakaiensis PETase with a fixed substrate-binding W156 residue

Abstract

The S92P/D157A variant of Ideonella sakaiensis PETase (IsPETase) showed significantly enhanced thermostability and PET degradation activity. The W156 residue of the variant was fixed in the substrate-binding conformation.