Efficient plant regeneration in vitro in buckwheat

Abstract

An in vitro highly efficient plant regeneration system was established from hypocotyl segments in buckwheat (Fagopyrum esculentum Moench.). Calli were induced on Murashige–Skoog (MS) medium containing 1.0 mg l−1 to 2.0 mg l−1 2,4-dichlorophenoxyacetic acid and 1.5 mg l−1 6-benzylaminopurine. Shoot buds were formed on subcultured pieces of callus. A high frequency (over 80%) of shoot differentiation was obtained on MS medium supplemented with 2.0 mg l−1 6-benzylaminopurine and 1.0 mg l−1 6-furfurylaminopurine. The regenerated shoots rooted readily on MS medium plus 0.2 mg l−1naphthaleneacetic acid and 0.2 mg l−1 indole butyric acid. The regenerated plantlets were acclimatized and successfully transferred to pots. Chromosome examination showed that the regenerated plants had normal chromosome number (2n=16).