Efficient nitrogen removal from mature landfill leachate in a step feed continuous plug-flow system based on one-stage anammox process

Abstract

A continuous plug-flow multistage anoxic/oxic (A/O) system based on one-stage partial nitrification coupled anammox (PNA) process with integrated fixed-film activated sludge (IFAS) was established and operated over 400 days. A step feed strategy effectively controlled free ammonia concentration and alleviated impacts on ammonia oxidizing bacteria (AOB) and anammox bacteria (AnAOB). During day 301–405, 98.1% of total inorganic nitrogen was removed from mature landfill leachate, whereas chemical oxygen demand (COD) removal efficiency was 52.9%. With the enrichment of AnAOB in oxic biofilm, nitrogen removal via the anammox pathway reached 94.3%-95.0%. During system operation, the dominant anammox genus shifted from Candidatus_Brocadia to Candidatus_Kuenenia. Fluorescent in situ hybridization (FISH) indicated AnAOB encapsulated by AOB colonies were mainly distributed inside of the biofilm, which promoted nitrite utilization by the anammox process. This innovative system and the results are of great value to practical applications.