Abstract
A rapid microwave-assisted extraction (MAE) method was established to extract commercially important salidroside from dry plant materials of Rhodiola crenulata. Several parameters such as extraction time, solid/liquid ratio, extraction temperature and solvent concentration were investigated for efficient salidroside extraction. The estimated maximum salidroside extraction ratio based on parameter optimization using response surface methodology (RSM) reached 2.17% (w/w) in 20 min at optimum conditions of 90 °C, 60% aqueous ethanol and solid to liquid ratio of 2 g/100 mL, which was confirmed to the experimental data. Compared with ultrasonic assistant extraction method (UAE) and heat reflux extraction method (HRE), MAE was the most efficient and effective method in the extraction of salidroside from the dry plant materials. The mechanism on the extraction enhancement using MAE was elucidated further using fourier transform infrared spectroscopy (FTIR) and light microscope.
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