Abstract
Effective propagation of protocorm-like bodies (PLBs) of Dendrobium sonia-28 was achieved in this study. The highest PLBs proliferation within 21 days was observed in semi-solid media over liquid culture. Half strength of Murashige and Skoog (MS) media produced the highest PLBs growth rate percentage compared to full and double strength MS media in semi-solid culture. In sucrose treatment, the highest increased of PLBs growth rate percentage was in 10g.L-1. PLBs growth rate percentage was the highest (14%) in media devoid of exogenous 6-benzylaminopurine (BAP) or naphthalene acetic acid (NAA). However, the addition of BAP and NAA in combinations of BAP (4.44 or 8.88 µM) and NAA (8.88 µM) resulted in increased PLBs growth rate to 14% compared to when added separately. The optimized micropropagation media and condition of PLBs proliferation are important for the production of large number of plant material required for the development of genetic modification and cryopreservation studies.
Highlights
Dendrobiums are highly valued in the flower industry as potted plant and cut flower (Khosravi et al, 2008)
Protocorm-like bodies (PLBs) were initiated aseptically by culturing nodal segment of Dendrobium sonia-28 in a semi-solid media and incubated at 25±2°C under 16 hours photoperiod
Effects of different Murashige and Skoog (MS) strengths on PLBs growth rate Growth rate percentage of more than 12% were recorded for protocorm-like bodies (PLBs) cultivated in half strength MS in semi-solid media (Figure 1a)
Summary
Dendrobiums are highly valued in the flower industry as potted plant and cut flower (Khosravi et al, 2008). Micropropagation provided an important breakthrough for mass propagation of many orchid species which have highly heterozygous genotype and have extremely slow sexual reproduction capability (Kanjilal et al, 1999). MS medium (Murashige and Skoog, 1962) widely used in many orchid’s micropropagation protocol including Dendrobium genus (Martin and Madassery, 2006; Fadel et al, 2010). Optimum media salt concentration is essential for in vitro orchid to provide sufficient nutrient require to promote metabolism and cell growth and to prevent toxic effect of media salt (Fadel et al, 2010). Sucrose has been accounted to be superior to other sugars for in vitro orchid growth (Jawan et al, 2010). Sucrose is available to cell and directly participate in glycolytic and penthose phosphate pathways for cell growth and was found to act as osmotic
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