Abstract
The CRISPR/Cas9 technology opens new avenues for detailed genetic exploration and comparative genomics. However, the current application of CRISPR/Cas9 in C. briggsae especially for homology-directed repair (HDR) knock-in (KI) still encounter significant challenges. In this study, we demonstrate that employing high concentration HDR donor vectors significantly improves KI efficiency in C. briggsae .
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