Abstract
An efficient in vitro regeneration technique was established in sugar beet (Beta vulgaris L.) using cotyledon as explants from in vitro raised seedlings. Regarding plant growth regulators added in inoculation media showed that BA in combinations with NAA was most responsive for multiple shooting in which, 90% of shoot emergence from inoculated explants were observed with a medium containing 0.5mg/L BA+0.5mg/L NAA at 30 days of inoculation period. The highest number of shoots per explant was 7.5? 0.56 and the maximum shoot length of 6.5?0.25cmwere observed in this medium inoculation after 90 days. In vitro raised shoots rooted well when they were transferred to MS + 3.0mg/LIBA in which, 70% shoots were rooted in this medium transferring after 30 days. The maximum number of roots per shoot was 5.50?0.46 and the average root length of 5.8 ?0.62 cm were recorded inoculation after 45 days. About6 months were needed from culture initiation to plantlets establishment in natural environment and 55% plantlets were found to be acclimatized and resumed a new growth.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Agriculture and Environmental Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
