Efficient In vitro Mass-propagation of Shiode(Smilax oldhami Miq.) through Liquid Culture.

Abstract

Shiode is a wild and edible plant whose flavor is similar to that of asparagus (Asparagus officinalis L.). The present experiments were implemented to improve the in vitro propagation system of Shiode. Internodal explants were cultured to derive protocorm-like bodies (PLBs) on Murashige and Skoog (MS) medium at pH 5.7. The MS medium was supplemented with 30g/l sucrose, 0.1mg/l α-naphthaleneacetic acid (NAA) and 0.1mg/l 6-benzylaminopurine (BAP), and solidified with 8g/l agar. The PLBs that were induced on the solid MS medium propagated very rapidly when they were transferred into the 1/2 MS liquid medium at pH 5.7 supplemented with 20g/l sucrose, 1.0mg/l NAA and 0.1 or 1.0mg/l BAP. The liquid MS medium was renewed periodically. The single PLBs were separated from the mother PLB-clusters by a surgical knife, and were used to regenerate the descendant PLB-clusters. The secondary PLBs grew adventitious shoots that formed roots on the solid hormone-free MS medium after subculture for 2 weeks on the solid MS medium supplemented with 0.5, 1.0 and 2.0mg/l NAA. The plantlets were acclimatized following standard procedures.