Abstract
Efficient Protein Synthesis In article 2200023, Qi and co-workers show that by orthogonal degradation of the targeted protein, both essential and alternative termination types of machinery are completely removed from E. coli cell extract. Furthermore, a total of 153 engineered tRNAs are screened for efficient all stop-codons decoding to construct a codon-dependent termination defect in vitro protein synthesis with all 64 sense-codons, iPSSC, which holds potential in building artificial protein synthesis beyond the cell.
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