Efficient Hybridization between Lilium*elegance and L. longiflorum through in vitro Ovary Slice Culture.

Abstract

Successful hybridization between Lilium×elegance and L. Ionglflorum has not been reported, although its reciprocal cross has been developed. Ovary slice culture technique, after cut-style pollination, was used to produce interspecific hybrids between these species. Crosses between diploid cultivars (2n=2x=24) of Lilium X elegance and L. Ionglflorum were conducted. On the days 5, 10, 15, 20, 25, 30, 35, 40, and 45th after pollination (DAP) ovaries were sliced and cultured on a modified MS (Murashige and Skoog 1962) medium without growth regulators and ammonium nitrate (NH4N03), supplemented with 6% sucrose, 50 mg/l yeast extract and 0.25% gelrite at pH 6.3. Embryo germination was found only at 35 DAP. After transfer to a MS (half-strength) medium supplemented with 1.5% sucrose, 0.25% gelrite and 0.2% active charcoal at pH 5.8, seven hybrid plants were developed. All regenerated plants were identified as hybrids on the basis of karyotype, isozyme and PCR analyses.