Efficient enrichment and flow sorting of aldehyde dehydrogenase bright hematopoietic progenitor cells from thawed, banked umbilical cord blood

Abstract

Almost all of the hematopoietic progenitor cells that durably reconstitute NOD/SCID mice and the majority of long-term and short-term multilineage colony initiating cells in fresh human umbilical cord blood (UCB) can be isolated by flow-sortinglow-side scatter cells that express high levels of the enzymealdehyde dehydrogenase (ALDHbrSSClo). Such highly enriched ALDHbrSSClo cell populations may be useful in expansion of UCB stem cells and other clinical applications. These clinical preparations of UCB stem cells are likely to be derived from UCB that has been frozen, banked, and then thawed to manufacture a therapeutic preparation.