Efficient elimination and re-growth inhibition of harmful bloom-forming cyanobacteria using surface-functionalized microbubbles

Abstract

The elimination of cyanobacteria is frequently required for treating and controlling the waters with harmful algal blooms. In this study, an improved flotation technology was developed using colloidal gas aphrons (CGAs) surface-modified with the inorganic coagulant of polyaluminum chloride (PACl); the Microcystis aeruginosa (M. aeruginosa) cells were efficiently removed and their re-growth was effectively inhibited. The so-created coagulative CGAs (CCGAs) exhibited the attractive characteristics of both CGAs and PACl for the cell removal. The experimental results clearly showed that 94.2–99.2% of cells were removed within 3 min at the optimum dosage of cetyltrimethyl ammonium bromide (CTAB) and PACl at three different initial cell densities (OD680 = 0.05, 0.26 and 0.76); and the re-growth of M. aeruginosa did not occur in 10 days. The flocs derived from the CCGA-flotation were of smaller size and looser configuration in contrast with those obtained from coagulation-flotation. The CCGAs were robust in charge neutralization, cell capture, cell attack and destruction. Even at low CTAB dosages, those bubbles could provide large surface area for capturing the M. aeruginosa cells in both unicellular and colonial form compared with the unmodified CTAB-CGAs. The CCGAs reduced 59.5–87.9% of CTAB dosage with the assistance of PACl and the required flotation retention time was largely shortened in comparison with the sedimentation and flotation-based treatment options. This would lead to low treatment cost and sludge production. The present work provides a novel insight into the development of flotation technologies for treating and controlling dense harmful algal blooms.