Abstract
Emblica officinalis is a medicinal plant and distributed throughout the lower ranges of Himachal Pradesh. Its ph is low and has high amount of polysaccharides, polyphenols several pigments and other secondary metabolites. A slight modification of Warude et al. (2003) protocol for DNA isolation yielded good quality high molecular weight DNA free of contaminants from eight plants taken for analogy using RAPD as a potential marker. 120 mgPVP/g of tissue was used and the isolated DNA pellets were washed twice with 70% ethanol. Key words: Himachal Pradesh, medicinal plant, Emblica officinalis,polysaccharides, PCR, RAPD, PVP.
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