Efficient determination of the p Ka values of six chlorinated phenols by reversed-phase liquid chromatography

Abstract

A non-linear regression algorithm is used to compute acidity constants from capacity factors recorded at various pH values using a standard reversed-phase column and acetonitrile as organic modifier. An application is presented for six chlorophenols and the p K a values calculated for those compounds compare favourable with literature data obtained by other methods in aqueous media. This p K a determination method is also suitable for mobile phase optimization; a resolution map based on p K a values from the literature and liquid chromatography-related p K a values is presented.