Abstract
Cane molasses is one of the main by-products of sugar refineries, which is rich in sucrose. In this work, low-cost cane molasses was introduced as an alternative substrate for isomaltulose production. Using the engineered Yarrowia lipolytica, the isomaltulose production reached the highest (102.6 g L−1) at flask level with pretreated cane molasses of 350 g L−1 and corn steep liquor of 1.0 g L−1. During fed-batch fermentation, the maximal isomaltulose concentration (161.2 g L−1) was achieved with 0.96 g g−1 yield within 80 h. Simultaneously, monosaccharides were completely depleted, harvesting the high isomaltulose purity (97.4%) and high lipid level (12.2 g L−1). Additionally, the lipids comprised of 94.29% C16 and C18 fatty acids, were proved suitable for biodiesel production. Therefore, the bioprocess employed using cane molasses in this study was low-cost and eco-friendly for high-purity isomaltulose production, coupling with valuable lipids.
Highlights
IntroductionCane molasses is one of the main by-products of sugar refineries, which contains saccharides (primarily sucrose, glucose and fructose) and a small amount of nitrogenous compounds, vitamins, and trace metal elements as well as colloids [1,2]
Cane molasses is one of the main by-products of sugar refineries, which contains saccharides and a small amount of nitrogenous compounds, vitamins, and trace metal elements as well as colloids [1,2]
Sucrose in cane molasses can be hydrolyzed into monosaccharides by microbial enzymes, which is believed to be suitable for microbial growth and metabolite production [8,13]
Summary
Cane molasses is one of the main by-products of sugar refineries, which contains saccharides (primarily sucrose, glucose and fructose) and a small amount of nitrogenous compounds, vitamins, and trace metal elements as well as colloids [1,2]. Sugarcane grows worldwide and mainly distributes in Brazil, India, and China. The molasses production in China is around 400 million tons per year, yet, large volumes of waste molasses are discharged, contributing to severe environmental pollution [3]. Cane molasses can be used as an available source of quick energy for animal feed or feed supplement [4]. Given that available ingredients enrich in cane molasses, nowadays, it is increasingly utilized as an alternative feedstock for microbial fermentation after a pretreatment or as the raw material. Different high value-added metabolites have been harvested via microbial
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