Abstract
In this study, a newly isolated strain Filobasidium magnum JD1025 was investigated for its production of sclareolide, which was verified to be a valuable raw material in various industrial fields. Together with a comprehensive analysis of the genome sequence, effective fermentation method to convert sclareol to sclareolide via the isolated strain was explored and optimized by taking the selected co-solvent and nitrogen source into account. The results showed that the final conversion rate could be achieved at 88.79 ± 1.06% with the initial sclareol concentration of 30 g·L-1 after 72 h in baffled flask. The corresponding yield concentration of sclareolide was 21.62 ± 0.26 g·L-1 and the conversion rate per unit thallus attained to 6.11 ± 0.06 % g-1·L-1. Overall, the current study suggested a valid method for the application of Filobasidium magnum JD1025 as bio-transformer to produce sclareolide from sclareol.
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