Abstract
R-(-)-linalool is widely used in pharmaceutical, agrochemical, and fragrance industries. However, plant extraction furnishes only limited and unstable R-(-)-linalool yields that do not satisfy market demand. Therefore, a sustainable yet efficient and productive method is urgently needed. To induce the R-(-)-linalool biosynthesis pathway in Escherichia coli, we expressed several heterologous (3R)-linalool synthases (LISs) and then chose a suitable LIS from Streptomyces clavuligerus (bLIS) for further study. The bLIS expression was markedly elevated by using optimized ribosomal binding sites and protein fusion tags. To increase the geranyl diphosphate content, we tested various alterations in prenyltransferases and their mutants. The final strain accumulated 100.1 and 1027.3 mg L-1 R-(-)-linalool under shake flask and fed-batch fermentation conditions, respectively. The latter is the highest reported R-(-)-linalool yield to date. This work could lay theoretical and empirical foundations for engineering terpenoid pathways and optimizing other metabolic pathways.
Highlights
R-(-)-linalool is a versatile acyclic monoterpene alcohol with applications in the flavor and fragrance, pharmaceutical, and agrochemical industries
In this study, an efficient R-(-)-linalool production pathway was induced in E. coli via the heterologous MVA pathway, Abies grandis GPPS (AgGPPS), and (3R)-linalool synthase
We propose that the factors contributing to this deficiency include insufficient precursor supply, linalool toxicity, and inefficient linalool synthase (LIS)
Summary
R-(-)-linalool is a versatile acyclic monoterpene alcohol with applications in the flavor and fragrance, pharmaceutical, and agrochemical industries. A sustainable yet efficient and productive method of R-(-)-linalool synthesis is urgently needed. Linalool (3,7-dimethyl-1,6-octadien-3-ol) is an acyclic monoterpene tertiary alcohol and an important natural floral scent. In perfumery, ~70% of the floral scent terpenoids consist of linalool. The odor of this substance is described as “light and refreshing, floralwoody, with a faint citrusy note” [1]. Linalool is a commonly used fragrance and flavoring agent in household detergents, furniture care products, waxes, processed foods, and beverages. It is a critical intermediate in vitamin E synthesis. Increasing environmental awareness has prompted investigators to generate R-(-)-linalool by more sustainable methods
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
