Abstract

The luminescence of the bisdeoxy analogue of coelenterazine ( 1b : X=Y=H) catalyzed by Oplophorus luciferase was highly efficient (0.19 quanta/molecule), almost comparable to the luminescence of unmodified coelenterazine ( 1a : X=Y=OH), whereas the luminescence of 1b was very inefficient when catalyzed by apoaequorin or Renilla luciferase and when the aequorin regenerated with 1b was luminesced with Ca 2+. © 1997 Elsevier Science Ltd.

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