Efficient Biofuel Generation of Plants via Enzymes

Abstract

The purpose of this research is to observe the Efficiency of Biofuel Generation of Plants Using Enzymes. The question being asked is at which concentration of glucose will yeast fermentation rates be the most efficient in order to produce Ethanol through fermentation? It was predicted glucose concentrations and ethanol production rates would increase simultaneously. Ethanol is a renewable transportation fuel made from plants. Methods for researching this topic includes studying the environmental impacts of Ethanol, how it is produced, the process of enzyme digestion, the differences between cellulose and cellulase and the process of yeast alcohol fermentation. The methodology required for this experiment includes creating eight samples: Cellulase + Yeast, Cellulose + Yeast, Cellulase + Cellulose + Yeast, Cellulase + Cellulose + No yeast, and all four concentrations of glucose in separate samples (0.5%, 1%, 3% and 5%). The apparatus used to collect the data is made up of a syringe attached to a pipette, each sample started at a given volume of mL then as the samples began fermenting and releasing CO2 data was recorded for 15 minutes in 1 minute intervals.The volume of carbon dioxide released corresponds to the amount of sugar (glucose) fermented. The results of this experiment show a correlation between the increased rate of ethanol production as glucose concentrations increase. The interaction between cellulose and cellulase demonstrate the process of producing glucose through enzyme digestion.