Abstract
Removal of biofilms is extremely pivotal in environmental and medicinal fields. Therefore, reporting the new-enzymes and their combinations for dispersal of infectious biofilms can be extremely critical. Herein, for the first time, we accessed the enzyme “protease from bovine pancreas type-I (PtI)” for anti-biofilm properties. We further investigated the anti-biofilm potential of PtI in combination with α-amylase from Bacillus sp. (αA). PtI showed a very significant biofilm inhibition effect (86.5%, 88.4%, and 67%) and biofilm prevention effect (66%, 64%, and 70%), against the E. coli, S. aureus, and MRSA, respectively. However, the new enzyme combination (Ec-PtI+αA) exhibited biofilm inhibition effect (78%, 90%, and 93%) and a biofilm prevention effect (44%, 51%, and 77%) against E. coli, S. aureus, and MRSA, respectively. The studied enzymes were found not to be anti-bacterial against the E. coli, S. aureus, and MRSA. In summary, the PtI exhibited significant anti-biofilm effects against S. aureus, MRSA, and E. coli. Ec-PtI+αA exhibited enhancement of the anti-biofilm effects against S. aureus and MRSA biofilms. Therefore, this study revealed that this Ec-PtI+αA enzymatic system can be extremely vital for the treatment of biofilm complications resulting from E. coli, S. aureus, and MRSA.
Highlights
Bacteria demonstrates versatile-tactics to infect humans [1]
The biofilms of E. coli, S. aureus, and methicillin-resistant S. aureus (MRSA) were successfully established in 6-well plates
The pancreas type-I (PtI), α-amylase from Bacillus sp. (αA), and Ec-PtI+αA were accessed their potential for biofilm inhibition of E. coli, S. aureus, and MRSA
Summary
Bacteria demonstrates versatile-tactics to infect humans [1]. In acute infections, they promptly spread and proliferate as a planktonic/individual form [2,3]. When an infection reaches the persistent or chronic stage, they largely colonize the tissues and other body-surfaces in highly-organized patterns of multicellular-aggregates termed as biofilms [1,4,5]. The important strategy adopted by bacteria for survival against anti-microbial materials and hostile environmental conditions is the formation of a rigid biofilm [1,2,6]. The contamination of the medical device and food packaging surfaces with pathogenic bacteria might lead to the biofilm formation, thereby it can cause serious acute and chronic infections to people [8,9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
