Efficient bioelectricity generation and carbazole biodegradation using an electrochemically active bacterium Sphingobium yanoikuyae XLDN2-5

Abstract

Carbazole and its derivatives are polycyclic aromatic heterocycles with unusual toxicity and mutagenicity. However, disposal of these polycyclic aromatic heterocycles remains a significant challenge. This study focused on efficient resource recovery from carbazole using an obligate aerobe, Sphingobium yanoikuyae XLDN2-5, in microbial fuel cells (MFCs). S. yanoikuyae XLDN2-5 successfully achieved carbazole degradation and simultaneously electricity generation in MFCs with a maximum power density of 496.8 mW m−2 and carbazole degradation rate of 100%. It is the first time that S. yanoikuyae XLDN2-5 was discovered as an electrochemically active bacterium with high extracellular electron transfer (EET) capability. Redox mediator analysis indicated that no self-produced redox mediators were found for S. yanoikuyae XLDN2-5 under analysis conditions, and the exogenous redox mediators used in this study did not promote its EET. The nanowires produced by S. yanoikuyae XLDN2-5 cells were found in the biofilm by morphology characterization and the growth process of the nanowires was consistent with the discharge process of the MFC. Conductivity determination further verified that the nanowires produced by S. yanoikuyae XLDN2-5 cells were electrically conductive. Based on these results, it is speculated that S. yanoikuyae XLDN2-5 may mainly utilize conductive nanowires produced by itself rather than redox mediators to meet the requirements of normal energy metabolism when it grows in the low dissolved oxygen zone of the anodic biofilm. These novel findings on the EET mechanism of S. yanoikuyae XLDN2-5 lay a foundation for further exploration of polycyclic aromatic heterocyclic pollutants treatment in electrochemical devices, which may create new biotechnology processes for these pollutants control.