Abstract
By selective modification of serine 221 in the active site, the preceding protease subtilisin carlsberg gained peroxidase activity which was subsequently utilized for the enantioselective reduction of hydroperoxides. Thus, semisynthetic seleno-subtilisin selectively catalyzed the kinetic resolution of four racemic alkyl aryl hydroperoxides p-R 2-C 6H 4CH(OOH)R 1 (R 1=CH 3, CH 2OH, CH(CH 3) 2; R 2=H, Cl). The enantiomeric distribution of all products was determined and kinetic parameters were evaluated, indicating seleno-subtilisin as the first semisynthetic enzyme with catalytic efficiency comparable to native enzymes and good enantioselectivity. In order to facilitate further studies, structural motifs responsible for enantioselectivity and efficiency of catalysis were outlined in the discussion, thus providing a rational basis for the selection of suitable substrates.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.