Efficient Access to Nonhydrolyzable Initiator tRNA Based on the Synthesis of 3′‐Azido‐3′‐Deoxyadenosine RNA

Abstract

Flexibility exercised: Hydrolysis-resistant 3′-aminoacyl-tRNA conjugates that contain a stable amide linkage instead of the natural ester are valuable substrates for biochemical studies of ribosomal processes. In a novel preparation of the stable E. coli initiator tRNA derivative 3′-(N-formylmethionyl)amino-tRNAfMet the key feature is the synthesis of 3′-azido oligoribonucleotides using a new functionalized solid support.