Abstract
(3S)-Acetoin and (2S,3S)-2,3-butanediol are important platform chemicals widely applied in the asymmetric synthesis of valuable chiral chemicals. However, their production by fermentative methods is difficult to perform. This study aimed to develop a whole-cell biocatalysis strategy for the production of (3S)-acetoin and (2S,3S)-2,3-butanediol from meso-2,3-butanediol. First, E. coli co-expressing (2R,3R)-2,3-butanediol dehydrogenase, NADH oxidase and Vitreoscilla hemoglobin was developed for (3S)-acetoin production from meso-2,3-butanediol. Maximum (3S)-acetoin concentration of 72.38 g/L with the stereoisomeric purity of 94.65% was achieved at 24 h under optimal conditions. Subsequently, we developed another biocatalyst co-expressing (2S,3S)-2,3-butanediol dehydrogenase and formate dehydrogenase for (2S,3S)-2,3-butanediol production from (3S)-acetoin. Synchronous catalysis together with two biocatalysts afforded 38.41 g/L of (2S,3S)-butanediol with stereoisomeric purity of 98.03% from 40 g/L meso-2,3-butanediol. These results exhibited the potential for (3S)-acetoin and (2S,3S)-butanediol production from meso-2,3-butanediol as a substrate via whole-cell biocatalysis.
Highlights
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China; National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fax: +86-591-8378-9121 (L.Z.)
Α-Acetolactate synthase (α-ALS), α-acetolactate decarboxylase (α-ALDC), and 2,3-butanediol dehydrogenase (BDH) in natural producing strains have been revealed in previous studies as three
SDSanalysis demonstrated that three bands and Vitreoscilla hemoglobin (VHB) could analysis demonstrated thatclear three clearofbands ofoxidase, NADH(2R,3R)-2,3-BDH, oxidase, (2R,3R)-2,3-BDH, and be observed in three recombinant E. coli strains (Figure 2), implying that the nox, rrbdh, and vgb genes could be observed in three recombinant E. coli strains (Figure 2), implying that the nox, rrbdh, and vgb were successfully expressed or co-expressed in recombinant strains
Summary
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China; National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fax: +86-591-8378-9121 (L.Z.). Abstract: (3S)-Acetoin and (2S,3S)-2,3-butanediol are important platform chemicals widely applied in the asymmetric synthesis of valuable chiral chemicals. Their production by fermentative methods is difficult to perform. Maximum (3S)-acetoin concentration of 72.38 g/L with the stereoisomeric purity of 94.65% was achieved at 24 h under optimal conditions We developed another biocatalyst co-expressing (2S,3S)-2,3-butanediol dehydrogenase and formate dehydrogenase for (2S,3S)-2,3-butanediol production from (3S)-acetoin. Synchronous catalysis together with two biocatalysts afforded 38.41 g/L of (2S,3S)-butanediol with stereoisomeric purity of 98.03% from 40 g/L meso-2,3-butanediol. These results exhibited the potential for (3S)-acetoin and (2S,3S)-butanediol production from meso-2,3-butanediol as a substrate via whole-cell biocatalysis. Α-Acetolactate synthase (α-ALS), α-acetolactate decarboxylase (α-ALDC), and 2,3-butanediol dehydrogenase (BDH) in natural producing strains have been revealed in previous studies as three
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