Efficiency of using biostimulants Agrinos 1 and Agrinos 2 in adaptation of microplants of garden strawberry to ex vitro conditions

Marina Karpushina,Olesya Yaroshenko,Marina Winter,E Egorov,N Zaporozhets,E Yakimenko,I Ilina

doi:10.1051/bioconf/20213403006

Abstract

This article presents the results of experimental studies of the effect of biostimulants Agrinos 1 and Agrinos 2, containing strains of microorganisms Azotobacter vinelandii and Clostridium pasteurinum, etc., as well as a complex of nutrients (macro-, microelements, protein, amino acids, chitin, chitosan) on the activation of metabolic processes and reduction of stress effects on strawberry plants obtained by in vitro method. The effectiveness of the reaction of biostimulants on the adaptive processes of growth and development of microplants of garden strawberry varieties Alba, Kemiya, Clery has been studied. In the course of the research, a positive effect of biological products on the growth of the vegetative mass of plants was established: an increase in the height of the plants compared to the control by 20-25%, the number of leaves by 25-28% and an increase in the root system of plants by 30-40%. In the Clery variety, the use of the biostimulants promoted the formation of the runners. In addition, the introduction of biostimulants into the soil contributed to the improvement of the quality indicators of the soil. The content of nitrate nitrogen in the soil increased by 18%.

Highlights

Micropropagation of garden strawberries in vitro is becoming one of the most widely used methods in plant growing [1]
This article presents the results of experimental studies of the effect of biostimulants Agrinos 1 and Agrinos 2, containing strains of microorganisms Azotobacter vinelandii and Clostridium pasteurinum, etc., as well as a complex of nutrients on the activation of metabolic processes and reduction of stress effects on strawberry plants obtained by in vitro method
In the course of the research, a positive effect of biological products on the growth of the vegetative mass of plants was established: an increase in the height of the plants compared to the control by 20-25%, the number of leaves by 2528% and an increase in the root system of plants by 30-40%

Summary

Introduction

Micropropagation of garden strawberries in vitro is becoming one of the most widely used methods in plant growing [1]. One of the main and problematic stages in the process of obtaining healthy strawberry planting material using micropropagation methods is the transfer of rooted plants obtained in vitro to non-sterile ex vitro conditions. Plants grown in vitro are very delicate as the micropropagation process takes place under conditions of high humidity, controlled temperatures and low light intensity. In this regard, such plants lack protective mechanisms: waxy cuticle, stomata regulation, leaf hairs, which makes them very vulnerable to drying out under the influence of environmental conditions after plant transplantation from in vitro to ex vitro conditions [2]

Methods

Results

Conclusion