Efficiency of molecular detection of Toxoplasma gondii contrasting to serological method.

Abstract

Toxoplasmosis infection of the mother during pregnancy is linked to transplacental transmission to the fetus. Toxoplasma gondii infection is difficult to diagnose and necessitates a comprehensive examination that includes laboratory and clinical testing. This study used RT-PCR using B1 gene based on paired blood samples and clinical data, for the detection of Toxoplasma infections in miscarriage women, this technique was compared to enzyme-linked immunosorbent assay (ELISA). There are a total of 125 miscarriage women at Al-Muthanna Hospital. Anti-Toxoplasma antibodies, IgG (latent infection) and IgM (recently acquired infection), were positive in 50/125 cases, with 25.6% IgG, 8.8% IgM, and 5.6% for IgM and IgG. In addition, women who have had two miscarriages have a high seropositivity rate. Furthermore, the development of molecular technologies to amplify parasite nucleic acids has enhanced toxoplasmosis diagnosis. The cycle threshold values Ct, which indicate the target gene's quantity, were calculated. With DNA from 500 T. gondii tachyzoites, a Ct of 25-28 was usually obtained. Present results show that the assay's reproducibility was found in 50 which considered (40%) from total miscarriage women of ELISA positive samples while 28/125 samples (22.4%) will positively in molecular detection and 56% from serological test was positive in molecular test. A move forward was the invention of diagnostic methods that used ELISA IgG and IgM followed by an RT-PCR methods in women were the most effective in detecting recent and reactive Toxoplasma gondii infection.