Abstract

Genetic diversity of 27 Moroccan pomegranate cultivars from three geographical regions of Morocco was screened using 61 Inter-Simple Sequence Repeat (ISSR) markers. Eight tested ISSR primers generated 70 bands of which 61 are polymorphic (87.14%). Nei's genetic distance ranged from (0.12 to 1) with a mean of 0.67. Nei genetic diversity parameters were 0.35 and 0.32 for Ht and Hs, respectively. Our results show considerable gene flow within and among pomegranate groups and a limited differentiation among groups as suggested by values of Gst (0.08) and Nm (5.80) parameters. The Unweighted Pair Group Method with Arithmetic Mean dendrogram and Principal Component Analysis clustered the pomegranate studied into two main clusters and did not show any relationships with the geographical origins of cultivars. Our results showed that ISSR markers can be a useful tool for detecting molecular polymorphism and studying the genetic diversity of pomegranate genotypes.

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