Abstract
10 �5 -10 �4 for peptides and 10 �8 -10 �7 for matrices are far smaller than 10 �3 -10 �1 for peptides and 10 �5 -10 �3 for matrices speculated by Hillenkamp and Karas. Number of gas-phase ions generated by MALDI was unaffected by laser wavelength or pulse energy. This suggests that the main role of photo-absorption in MALDI is not in generating ions via a multi-photon process but in ablating materials in a solid sample to the gas phase.
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