Efficiency of FAME analysis with SP-2380 capillary column in lamb meat

Abstract

AbstractThe healthiness and flavour of meat are largely related to its fat content and fatty acids (FAs) composition. In ruminant tissues an important role is played by the isomers of octadecadienoic acids (the most abundant of which are cis9trans11 and trans11cis12) as well as the cis and trans isomers of C18:1w9 and C18:2w6. It is very useful separate them together with the other FAs methyl esters (FAMEs) by a single run, as performed by capillary column highly polar.In this work, GC/MS analyses were performed with a Varian system (Saturn 2100T) and the separation was achieved on a SP-2380 (Supelco, Bellafonte, PA, USA) with stabilised poly (90% biscyanopropyl/10% cyanopropylphenyl siloxane) as a phase, with L=60m, I.D. 0.25mm, df=0.20 _m. According to the best separation and fastest analysis times, the temperature program was the following: initial 50°C, hold 5 min, ramp at 10°C/min, final 240°C, hold 2 min (with hold 8 min at 230°C).The method allowed selective and simultaneous determination of a wide...