Abstract
Ion-exchange high-performance liquid chromatography (HPIEC) is a method of choice for the separation of crude solid-phase synthesized oligonucleotides. It shows peaks for target and error sequences and it is used for quantification of oligonucleotide synthesis. This paper presents a homeodynamic model of polymer-supported oligonucleotide synthesis and a numerical solution, which accounts for elution profiles obtained by HPIEC. The elution profiles are consistent with the model. The chain-length distributions for 30mer syntheses on different support materials were also studied and the probability moment analysis was applied as a quantitative measure of the differential relationship of fractal dimension. The support systems for chemical synthesis of short oligonucleotides are classified according to this relationship.
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