Efficiency Assessment Of PTF1 For Phosphorous Deficiency Tolerance In Rice

Abstract

Phosphorous (P) deficiency tolerant rice genotypes can accumulate higher amount of P even though plant-available P in soil is low. Gobindabhog, an aromatic Bengal landrace was registered as one of the P-deficiency tolerant genotypes which can accumulate P almost 27mg/plant when grown in P-deficient field. Popular rice cultivar, Shatabdi accumulates approximately 6mg/plant P when grown in same soil. Relative abundance of transcript of Phosphate Starvation Sensing Transcription Factor 1 (PTF1) gene was estimated from the roots of 15- days old seedlings, grown both in P-deficient and sufficient conditions. Expression of PTF1 showed up-regulation in Gobindabhog under P-deficient condition whereas it was down-regulated in Shatabdi. RIL and NIL population were developed by crossing P deficiency tolerant genotype Gobindabhog with susceptible Shatabdi. SSR marker linked with PTF1 gene was used for genotyping the RIL and NIL population. P-accumulation in shoot at the pre-flowering stage and yield attributing parameters were estimated from the two groups of RILs and NILs grown in P-deficient soil. Single Factor analysis ANOVA revealed that PTF1 allele from Gobindabhog significantly increases P-accumulation as well as yield. Consequently, SSR marker RM 8258, located 24.06 KB away from PTF1 gene, can be recommended as a functional marker for introgression of superior allele of PTF1 from Gobindabhog to other non-tolerant genotypes.