Efficiency Assessment and Verification of Lisinopril by Spectrophotometry

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The goal is to provide a UV approach that is easy to use, affordable, accurate, and requires less time to estimate the amount of lisinopril in pharmaceutical formulations in bulk. Even though HPLC techniques are recognized in pharmacopoeias, non-chromatographic techniques such simple coupling processes or chemical modifications of lisinopril were similarly effective. The approach is predicated on UV spectroscopic methodology. Pharmaceutical formulations containing lisinopril, an ACE inhibitor that is frequently used to treat heart failure and hypertension, require precise and effective analytical techniques for estimating its amount. This work developed and validated a UV spectrophotometric approach for the quantitative analysis of lisinopril in pharmaceutical formulations as well as bulk form. The technique takes advantage of lisinopril’s natural absorbance properties, with maximal absorption being seen at 223nm. A high correlation coefficient (r = 0.9997) was found in linearity studies done throughout a concentration range of 10-50µg/ml, showing excellent linearity and suitable for quantitative analysis. The International Conference on Harmonization’s (ICH) guidelines were followed during the method’s validation. A detailed evaluation was conducted on a number of parameters, including specificity, robustness, ruggedness, accuracy, and precision (including repeatability and intermediate precision). The method’s accuracy and dependability were validated by validation study results, which fulfilled the exacting standards needed for pharmaceutical analysis. The suggested method’s accuracy was further supported by recovery trials, which showed that it could precisely quantify lisinopril even in the presence of formulation excipients and contaminants. The method’s specificity was demonstrated by interference studies, which also guaranteed that co-existing chemicals frequently found in pharmaceutical formulations would cause little influence.