Efficacy of Water-Soluble Pearl Powder Components Extracted by a CO2 Supercritical Extraction System in Promoting Wound Healing.

Minting Liu,Huajun Ruan,Hu Zhou,Xian Jun Loh,Changming Tang,Yunlong Wu,Hongchen Guo,Guorui Zhang,Junjun Tao,Liang Tang

doi:10.3390/ma14164458

Abstract

Pearl powder is a biologically active substance that is widely used in traditional medicine, skin repair and maintenance. The traditional industrial extraction processes of pearl powder are mainly based on water, acid or enzyme extraction methods, all of which have their own drawbacks. In this study, we propose a new extraction process for these active ingredients, specifically, water-soluble components of pearl powder extracted by a CO2 supercritical extraction system (SFE), followed by the extraction efficiency evaluation. A wound-healing activity was evaluated in vitro and in vivo. This demonstrated that the supercritical extraction technique showed high efficiency as measured by the total protein percentage. The extracts exhibited cell proliferation and migration-promoting activity, in addition to improving collagen formation and healing efficiency in vivo. In brief, this study proposes a novel extraction process for pearl powder, and the extracts were also explored for wound-healing bioactivity, demonstrating the potential in wound healing.

Highlights

IntroductionTrauma is a very common disease, which can be divided into two categories: acute wounds (surgery, trauma, abrasions and burns) and chronic wounds (diabetic foot ulcers) [1]
Trauma is a very common disease, which can be divided into two categories: acute wounds and chronic wounds [1]
The protein concentration was measured by Bicinchoninic Acid (BCA) to obtain the protein content in the extracts as 11.06 μg/10 g pearl powder (SFEPE) and 5.40 μg/10 g pearl powder, respectively

Summary

Introduction

Trauma is a very common disease, which can be divided into two categories: acute wounds (surgery, trauma, abrasions and burns) and chronic wounds (diabetic foot ulcers) [1]. Chronic wounds caused by chronic diseases such as diabetes or peripheral vascular disease can lead to abnormal wound healing. After acute injury (such as peeling or large-scale thermal injury from surgery), the function of the skin organ is lost, which makes organisms susceptible to infection, thermal disorders and fluid loss [2]. Wound healing embodies a dynamic and instantaneous reaction of the body to tissue injury with the objective of repairing anatomical continuity, structure and function. The success or failure of this complex cascade of events is defined largely by the competence of the host’s immune system. Sepsis exemplifies one of the most challenging risks to effective wound healing

Results

Discussion

Conclusion