Abstract
<p>Introduction. The amniotic membrane is used in transplant surgery, ophthalmology and dermatology. Various methods have been developed to preserve amniotic membrane: hypothermic storage, cryopreservation, lyophilization. Transplantation of fresh amniotic membrane showed low inflammatory response. The efficient antibiotic solutions are carefully chosen for the hypothermic storage of amniotic membranes. The aim of this study was to compare the efficacy of two antibiotic solutions for the hypothermic amniotic membrane preservation and the structure of the amniotic membrane after the preservation process. Methods. Fifteen amniotic membranes were prepared and hypothermically stored in penicillin solution in BSS (2000 IU/ml) while the remaining fifteen in an antibiotic solution containing: benzylpenicillin (50 µg/ml), gentamicin (100 µg/ml), ciprofloxacin (200 µg/ml) and fluconazole (100 µg/ml). All amniotic membranes were microbiologically tested after preparation and after hypothermic storage for two weeks. Histological analysis of thirty amniotic membranes was performed after the process of preservation. Results. Fifteen amniotic membranes were sterile after hypothermic preservation in the penicillin solution. Also, fifteen amniotic membranes were sterile after hypothermic preservation in the solution of antibacterial agents (penicillin, gentamicin, ciprofloxacin) and antifungal agent fluconazole. The amniotic membrane had a normal structure and thickness of 35.33±11.03 mm. Conclusion. Both antibiotic solutions, one that contains only penicillin and one that contains multiple antibacterial agents and fluconazole, provide sterility of fresh hypothermically stored amniotic membranes for two weeks. In the preparation of fresh hypothermically stored amniotic membrane, a solution with multiple antibiotics is preferred. The normal tissue structure of the amniotic membrane was histologically confirmed after the process of preservation.</p>
Highlights
The placenta is a temporary organ used for the development and growth of the fetus and provides the transfer of the oxygen and nutrients from the mother’s organism
The normal tissue structure of the amniotic membrane was histologically confirmed after the process of preservation
24 www.biomedicinskaistrazivanja.mef.ues.rs.ba of amniotic membranes, which were taken after amnion processing and before its packaging in sterile containers, bacteria and fungi were not isolated by microbiological testing (Table 1)
Summary
The placenta is a temporary organ used for the development and growth of the fetus and provides the transfer of the oxygen and nutrients from the mother’s organism. It consists of the maternal and the fetal part. Amnion is an extraembryonic membrane that participates in the construction of the fetal part of the placenta. It represents the innermost layer of the placenta and completely envelops. The amnion overlies the chorion and, by its separation from the chorion, an amniotic membrane is produced - AM. The thickness of the amniotic membrane is between 20-50 μm and it is composed of three layers: a single epithelial layer, abasement membrane and stroma which is further subdivided into compact, fibroblast and spongy layer [3]
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