Efficacy of sweep ultrasound on natural microbiota reduction and quality preservation of Chinese cabbage during storage

Abstract

In this study, the effect of sweep frequency ultrasound (SFUS), sodium hypochlorite (NaOCl) and their combinations (SFUS + NaOCl) in reducing and inhibiting natural microbiota as well as preserving quality of fresh-cut Chinese cabbage during storage (4 °C and 25 °C) for up to 7 days was investigated. In effect, 40 kHz sweep frequency ultrasound in combination with 100 mg/L sodium hypochlorite resulted in maximum reduction and inhibition of mesophilic counts, yeast and molds and minimum chlorophyll depletion, weight loss and electrolyte leakage. However, colour and textural characteristics deteriorated. The combined treatment suppressed the activities of polyphenol oxidase and peroxidase and manifested its preservative effect after Fourier Transform near-infrared spectroscopy analysis. Synergistic reductions were recorded in most of the combined treatments though largely <1.0 log CFU/g. Specifically, the combined treatment significantly (P < 0.05) reduced mesophilic counts by an added 2.7 log CFU/g, yeasts and molds by an added 2.0 log CFU/g when compared to the individual treatments. During storage at 4 and 25 °C, washing with SFUS + NaOCl produced Chinese cabbage with lower microbial counts, in comparison with the individual treatments. However, post-treatment storage could not entirely inhibit microbial survival as populations increased during storage even at refrigeration temperature of 4 °C. The results demonstrate that ultrasound and sodium hypochlorite are promising hurdle alternatives for the reduction and inhibition of microorganisms, as well as prolonging the shelf life and retaining the quality characteristics of Chinese cabbage.