Abstract
Efficacy of individual and mixed substrates i.e. rice bran, wheat bran, grass pea bran and their combination with mustard oilcake (MOC), based Trichoderma harzianum bio-fungicides were tested against seedling disease (Fusarium oxysporum) of brinjal in plastic tray and seed bed during 2009 to 2014 in the net house of Bangladesh Agricultural Research Institute (BARI). The results of plastic tray and seed bed experiments revealed that four combinations of substrate based T. harzianum bio-fungicides such as (1) rice bran + wheat bran, (2) rice bran + wheat bran + mustard oil cake (MOC), (3) rice bran + grasspea bran, and (4) rice bran + grasspea bran +MOC were suitable for controlling the soil borne seedling disease (F. oxysporum) of brinjal in tray soil as well as seed bed soil conditions.
Highlights
Bangladesh is yet to be self-sufficient in quality food and nutrition which are the major challenges to achieve a healthy and prosperous country like other developing countries of the world
Materials and Methods One experiment was conducted in the plastic tray and three experiments were conducted under seed bed conditions in the net house of Plant Pathology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur during the period 2009 to 2014 to find out the suitable substrate material for mass culturing of T. harzianum and thereby formulation of an effective bio-fungicides to control soil borne seedling disease (F. oxysporum) of brinjal
The efficacy of T. harzianum bio-fungicides against F. oxysporum causing germination failure and mortality of brinjal seedlings was tested in a plastic tray experiment where the soil was artificially inoculated with the pathogen F. oxysporum and treated with the different substrates based T. harzianum bio-fungicides
Summary
Bangladesh is yet to be self-sufficient in quality food and nutrition which are the major challenges to achieve a healthy and prosperous country like other developing countries of the world. Application of chemical fungicides is expensive and hazardous to health and environment (Brown and Hendrix 1980, Punja et al 1982) The beneficial microbes such as Trichoderma harzianum has been reported as a bio-control agent that effectively controlled the soil borne pathogens (Elad et al, 1983; Roy, 1989). Efficacy of Substrate Based Trichoderma Harzianum Bio-Fungicides in Controlling Seedling Disease (Fusarium Oxysporum) of Brinjal in Seed Bed pongamia (Shamarao et al, 1998). All of these substrate materials are available in Bangladesh but their potentialities to use in the formulation of T. harzianum bio-fungicide have not yet been studied in the country. The present study was undertaken to find out the effective local substrates to formulate the suitable medium for mass culturing of T. harzianum to be used as effective bio-fungicides against F. oxysporum causing germination failure and seedling mortality of brinjal under seed bed condition
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