Abstract
We present monitoring of nitrilase enzyme assay in real-time by means of Spectroscopic Optical Coherence Tomography (sOCT). Combination of principal component analysis (PCA) algorithm with Swept Source Optical Coherence Tomography (SSOCT), to extract the absorption spectra of the sample, termed as sOCT. Spectroscopic information of nitrilase enzyme obtained from sOCT is used to investigate dynamic enzyme kinetic reactions. Different principle components give complete information regarding the composition of the samples. Further, Fourier Transform Infrared Spectroscopy (FTIR) analysis has been also performed to validate the PCA absorption spectra. Samples were prepared in controlled condition and evaluated at a regular time interval of the incubation period. The developed algorithm and system are combinedly capable of in-vitro real-time, study of enzyme kinetics with respect to time as biosensor.
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