Abstract
ABSTRACT Anaesthetics in aquaculture serve as a stress avoidance mechanism, mitigating stress-related impacts during fish handling and transportation. This study assessed the efficacy of sodium bicarbonate and clove stem powder as anaesthetic agents for Nile Tilapia juveniles. Four hundred and twenty uniform O. niloticus juveniles (5 ± 0.5 g, 74 ± 5 mm) were exposed to varying concentrations of sodium bicarbonate (10, 15, 20, 25, 30, 35, 40 g/L) and clove stem powder (0, 1, 3, 5, 7, 10, 15 g/L) in 20 L transparent plastic tanks. The results showed an inverse relationship between anaesthetic concentration and induction time, a direct relationship with recovery time, and an inverse correlation between induction and recovery times for both agents (P < 0.001). The Appropriate concentrations were determined as 25 g/L for sodium bicarbonate and 10 g/L for clove stem powder. At these levels, induction and recovery times met ideal criteria (3-5 min induction, 5-10 min recovery), with fish exhibiting normal behavior and 100% survival after a week of monitoring.
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