Efficacy of Pyrus elaeagnifolia subsp. elaeagnifolia in acetic acid–induced colitis model

Mert Ilhan,Esra Küpeli Akkol,Ibrahim Tümen,Hakkı Taştan,Fatma Tuğçe Gürağaç Dereli

doi:10.1515/chem-2019-0002

Abstract

AbstractIn Turkish folk medicine, the fruits ofPyrus elaeagnifoliasubsp.elaeagnifoliahave been used to treat diarrhea and detoxify poisonous snake bites by enlarging the wound. The aim of the study was to confirm the ethnopharmacological usage of the plant usingin vivoandin vitromodels. Experimental colitis was performed under anesthesia by intrarectal administration of acetic acid in rats, and the extracts were administered orally. The colonic malondialdehyde (MDA), tumor necrosis factor (TNF-α), interleukin-6 (IL-6), and nitrite levels, in addition to the myeloperoxidase (MPO) and caspase-3 activities, were measured to determine the effects of the plant extracts. The methanol (MeOH) extract revealed a significant decrease in MPO and caspase-3 levels. The MeOH extract was found to have the highest total tannin content. It was also found to have significant antioxidant (p˂ 0.01) and anti-inflammatory activities (p˂ 0.05) in acetic acid induced colitis rat model . According to our results, the present study exhibited a decrease in MDA, nitrite, IL-6, and TNF-αlevels in the colon tissue and blood in the MeOH extract treated group. The findings of this study can help in treating various disorders, such asClostridium difficileinfection, irritable bowel syndrome, and inflammatory bowel diseases.

Highlights

The Pyrus L. species (Rosaceae) is the most economically important species in the Old World, from Western Europe and North Africa to Asia
The MeOH extract could cause a decrease in MPO activity, which could be considered as a result of the anti-inflammatory activities of the samples in the acetic acid model
Serum nitrite level was increased from 1.6 ± 0.2 to 1.8 ± 0.1 μg (p < 0.05) whereas the serum MDA level was decreased from 8.9 ± 3.4 to 4.1 ± 1.1 nmol/mL plasma (p < 0.01) with the treatment of the MeOH extract (Table 1)

Summary

Introduction

The Pyrus L. species (Rosaceae) is the most economically important species in the Old World, from Western Europe and North Africa to Asia. Some pear species are cultivated in Asia and Europe [1]. Nine species are growing in Turkey, namely, Pyrus boissieriana subsp. One of the main centers of the wild pear (Pyrus elaeagnifolia) is Anatolia and the plant is called as “ahlat” or “çördük” in Turkish [3,4]. Two additional subspecies are P. elaeagnifolia subsp. The fruits of the plant are one of the most highly consumed fresh or dried fruits in the world. The fruits of this species have splendid taste and aroma [5]

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