Efficacy of Platelet-Rich Plasma Containing Xenogenic Adipose Tissue-Derived Stromal Cells on Restoring Intervertebral Disc Degeneration: A Preclinical Study in a Rabbit Model.

Chao Ma,Shichong Wang,Lijuan Lu,Ying Han,Ran Wang,Tianyun Gao,Bin Wang,Naikun Wang,Dingliang Zhao

doi:10.1155/2019/6372356

Chao Ma, Shichong Wang + Show 7 more

Open Access

https://doi.org/10.1155/2019/6372356

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Journal: Pain research & management	Publication Date: Apr 16, 2019
Citations: 10	License type: CC BY 4.0

Affiliation: Nanjing Drum Tower Hospital

Abstract

Objective Platelet-rich plasma (PRP) containing multiple growth factors is a promising strategy for disc degeneration. Thus, this study hypothesizes that the combination of PRP and adipose tissue-derived stromal cells (ADSCs) may repair degenerative disc more effectively than using each one of them alone. Methods The model of early intervertebral disc degeneration was induced by annular puncture in the New Zealand rabbit. Autologous PRP was extracted from fresh arterial blood by using two centrifugation techniques. ADSC was offered by the Center for Clinic Stem Cell Research. Four weeks after the first experiment, PRP or ADSCs or a combination of PRP and ADSCs was injected into the punctured intervertebral disc. Four weeks later, disc height and signal intensity on T2-weighted magnetic resonance imaging (MRI) were assessed. Results One month after puncture, we detected relatively narrow discs and lower signal intensity in MRI T2-weighted images. At four weeks after injection, the PRP-ADSC group statistically significantly restored discs, compared with PRP, ADSCs, or negative control group. Conclusions The combination of PRP and ADSCs shows an effective potential to restore degenerated intervertebral discs in the rabbit.

Highlights

Intervertebral disc degeneration (IDD) is the leading cause of low back pain (LBP), leading to disability and placing a heavy burden on society [1]
Platelet-rich plasma (PRP) has been proven to be an effective therapy for IDD in rats, rabbits, and sheep treated in a large number of experimental animal models [2, 8]
Compared with disc L2/3, the signal image of L3/4, L4/5, and L5/6 were significantly higher. ese results suggest that biological therapy including but not limited to PRP and adipose tissue-derived stromal cells (ADSCs) could promote the repair of disc regression

Summary

Objective

Platelet-rich plasma (PRP) containing multiple growth factors is a promising strategy for disc degeneration. us, this study hypothesizes that the combination of PRP and adipose tissue-derived stromal cells (ADSCs) may repair degenerative disc more effectively than using each one of them alone. Us, this study hypothesizes that the combination of PRP and adipose tissue-derived stromal cells (ADSCs) may repair degenerative disc more effectively than using each one of them alone. E model of early intervertebral disc degeneration was induced by annular puncture in the New Zealand rabbit. Four weeks after the first experiment, PRP or ADSCs or a combination of PRP and ADSCs was injected into the punctured intervertebral disc. Disc height and signal intensity on T2-weighted magnetic resonance imaging (MRI) were assessed. We detected relatively narrow discs and lower signal intensity in MRI T2-weighted images. At four weeks after injection, the PRP-ADSC group statistically significantly restored discs, compared with PRP, ADSCs, or negative control group. E combination of PRP and ADSCs shows an effective potential to restore degenerated intervertebral discs in the rabbit Conclusions. e combination of PRP and ADSCs shows an effective potential to restore degenerated intervertebral discs in the rabbit

Introduction

Materials and Methods

Results