Abstract
The present study was performed on Holstein bulls at the Department of Artificial Insemination pertaining to the Directorate of Animal Resource, Ministry of Agriculture, Abu-Ghraib, Baghdad, during the period from December 2021 to June 2022. The experiments were conducted to improve low semen quality by addition of Nigella sativa aqueous extract to semen Tris extender. Four bulls, 3-5 years old and 500-750 Kg live body weight were trained to semen collection using artificial vagina (AV) weakly bad pooled fresh semen was divided into four groups. First group using as a control containing Tris extender only (C), second group added 0.5% NS extract, third group added 2% NS extract, forth group added 4% NS extract.
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