Abstract
Photodynamic therapy (PDT) is an emerging therapeutic procedure suitable for the treatment of cervical cancer. However, the side effects of PDT are severe, including skin ulceration, so we designed an experiment to examine the effects of multiple low- dose photodynamic therapy of 5, 10, 15, 20-tetrakis(1- methylpyridinium-4-yl) porphyrin (Tmpyp4) on tumour growth by utilizing a model in nude mice implanted with Hela cervical cancer cells. Female BALB/c nude mice (aged 5-6 weeks, weighing 18-20 g) were used. Hela cervical cancer cells were injected subcutaneously (1 x 10(7) cells/200 μL). Ten days after injection, the mice were divided into three groups (n=6), the A group of controls without any treatment, the B group receiving a single-treatment with Tmpyp4 (10 mg/kg, intratumor injection) and irradiation (blue laser, 108 J/cm(2)), and the C group given three-treatments with Tmpyp4 (10 mg/ kg, intratumor injection) and irradiation at intervals of two days. After starting treatment, tumours were measured every two days, to assess growth. At 2 weeks after the last treatment of C group, tumour tissue and organs were collected from each mouse to evaluate tumor histology and organ damage. Tumour growth in C group was significantly inhibited compared with A and B groups (P <0.05), without any injury to the skin and internal organs. Our novel findings demonstrated that multiple low-dose photodynamic therapy of Tmpyp4 could inhibit cervical cancer growth significantly with no apparent side effects.
Highlights
In recent years, the widespread screening of cervical cancer, especially the systemic use of the Papanicolaou (Pap) smear (Cronje, 2005), cervical cancer remains the second most common cause of death from cancer in women worldwide (Sreejata et al, 2012), China accounts for 29% of the 51,000,000 new cases of cervical cancer each year (Kim et al, 2009)
The side effects of Photodynamic therapy (PDT) are severe, including skin ulceration, so we designed an experiment to examine the effects of multiple low- dose photodynamic therapy of 5, 10, 15, 20-tetrakis(1methylpyridinium-4-yl) porphyrin (Tmpyp4) on tumour growth by utilizing a model in nude mice implanted with Hela cervical cancer cells
Some research suggested that the extent of photocytotoxicity after PDT is dependent on Observation on side-effects of multiple low-dose the photosensitizer type, the medication dose, the type of photodynamic therapy of Tmpyp4 tumor, the light fluence rate and the total light exposure
Summary
The widespread screening of cervical cancer, especially the systemic use of the Papanicolaou (Pap) smear (Cronje, 2005), cervical cancer remains the second most common cause of death from cancer in women worldwide (Sreejata et al, 2012), China accounts for 29% of the 51,000,000 new cases of cervical cancer each year (Kim et al, 2009). The side effects of PDT are severe, including skin ulceration, so we designed an experiment to examine the effects of multiple low- dose photodynamic therapy of 5, 10, 15, 20-tetrakis(1methylpyridinium-4-yl) porphyrin (Tmpyp4) on tumour growth by utilizing a model in nude mice implanted with Hela cervical cancer cells.
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