Efficacy of Moringa oleifera Lam. extracts and Pediococcus pentosaceus, Lactobacillus acidophilus, Lactobacillus plantarum probiotic during starter period on growth performance of male broiler chicken

Abstract

Background: Probiotics and medicinal plants have been used to support human and livestock health. This research aimed to evaluate the potential of Moringa oleifera Lam. leaf extract on the growth of Pediococcus pentosaceus, Lactobacillus acidophilus and L. plantarum probiotic in vitro and to prove its potential as a feed additive, as an alternative to antibiotic growth promoters (AGP) to improve nutrient intake, body weight gain, feed conversion ratio and feed efficiency in broiler chicken starter phase. Methods: This study consisted of three sub studies: (1) Screening test for phytochemical compounds; (2) evaluation of several doses of Moringa extract (0%, 0.1%, 0.2%, 0.3% ). At the end of treatment, each colony was measured using the total plate count; (3) evaluation of probiotics and M. oleifera in vivo to prove growth performance on starter-phase broiler chicken. All results were analyzed by analysis of variance (ANOVA) then followed by the Duncan test. Results: (1) The phytochemical screening test M. oleifera extract contained flavonoids, saponins, tannins, triterpenoids and alkaloids; (2) M. oleifera extract at doses of 0.1%, 0.2%, 0.3% increased the growth of bacteria Pediococcus pentosaceus, Lactobacillus acidophilus and Lactiplantibacillus plantarum (p<0.05); (3) The use of probiotics, M. oleifera extract and their combination showed that body weight, body weight gain, feed conversion rate (FCR) and feed efficiency signifcantly differed (p <0.05) between the treatments, but there was no significant difference (p > 0.05) between the treatments on nutrient intake (dry matter, ash, crude protein, crude fiber and organic matter) on broiler chicken starter phase. Conclusions: The use of M.oleifera extract at doses of 0.1%, 0.2% and 0.3% increased the growth of P. pentosaceus, L. acidophilus and L. plantarum bacteria in vitro and the use of probiotics, M.oleifera extract and their combination by in vivo improved the growth performance on starter phase of broilers chicken.