Abstract

The tomato leaf miner Phthorimaea absoluta(Meyrick), earlier known by the scientific name Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most devastating global pests of tomatoes. Using indigenous entomopathogenic fungi can be a promising solution to manage it. Effıcacyof EPF isolates on third-instar larvae was carried out in Ethiopia during 2021-2022. Ten larvae were inoculated with conidiaa suspensions with three replications. The result showed that larval mortality was significantly different (p<0.05) among concentrations and isolates. Beauveria bassiana isolates caused 70 to 81% mortality, the lowest LC 50 was 1.87x104 to 9.79x10 4 conidia/ ml; and LC 95 was 5.68x108 to 1.10x10 11 conidia/ml and shortest LT 50 was 3.6 to 5.7 days. These were considered highly virulent while Metarhizium anisopliae determined as moderately virulent having 46 to 63% mortality (highest LC50 was 1.36x106 to 4.94x10 7and LT 50 was 4.3 to 8 days). Results also revealed 1x10 10 conidia/ ml is the most effective dose while 1x10 6 is the least effective.

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